Eppendorf FemtoJet 4x v.1 Bedienungsanleitung

nualEN)g manual Register your instrument! www.eppendorf.com/myeppendorfFemtoJet® 4iOperating manual

Product descriptionFemtoJet® 4iEnglish (EN)102.4 Main illustrationAbb. 2-2:Front and rear sideFig. 2-2: Front and rear side1Display2 Control panel3 Po

Product descriptionFemtoJet® 4iEnglish (EN)112.4.1 InterfacesAbb. 2-3:InterfacesFig. 2-3: Interfaces1 Mains/power connection2Mains/power switch On/Off

Product descriptionFemtoJet® 4iEnglish (EN)122.5 Name plateAbb. 2-4:Name plateFig. 2-4: Name plate1 Manufacturer2Product number3Voltage4Micro fuse5Fre

Product descriptionFemtoJet® 4iEnglish (EN)132.6 Control panelThe keys of the control panel are used to switch on the Microinjector, to perform functi

Product descriptionFemtoJet® 4iEnglish (EN)142.7 Rotary knobsThe rotary knobs are used to set the injection parameters as injection time, injection pr

Product descriptionFemtoJet® 4iEnglish (EN)152.8 Foot controlYou can connect the foot control to the Microinjector.The foot control corresponds to the

Product descriptionFemtoJet® 4iEnglish (EN)162.10.1 Compensation pressure pcThe compensation pressure prevents the liquid from rising from the Petri d

Product descriptionFemtoJet® 4iEnglish (EN)172.10.4 Rinsing pressure The rinsing pressure is used to clean the capillary.2.10.5 Operating pressure pwT

SafetyFemtoJet® 4iEnglish (EN)183Safety3.1 Intended useThe FemtoJet 4i Microinjector is designed and manufactured for the exclusive use within the con

SafetyFemtoJet® 4iEnglish (EN)193.3 Warning signs on the device3.4 User profileThe device and accessories may only be operated by trained and skilled

5252 900.014-00/012014Copyright © 2014 Eppendorf AG, Hamburg. All rights reserved, including graphics and images. No part of this publication may be

InstallationFemtoJet® 4iEnglish (EN)204 Installation4.1 Preparing installation1. Check the packaging for damage.2. Check that everything is included i

InstallationFemtoJet® 4iEnglish (EN)214.4.1 Connecting a micromanipulatorPrerequisites• Y-cable FJ4 is available.• The Microinjector is switched off.•

InstallationFemtoJet® 4iEnglish (EN)224.4.3 Connecting two devicesPrerequisites• Y-cable FJ4 is available.• Devices are switched off.Two devices can b

SoftwareFemtoJet® 4iEnglish (EN)235 Software5.1 Main screenThe main screen displays all injection parameters, the selected pressure unit, the injectio

SoftwareFemtoJet® 4iEnglish (EN)245.2 Main menuAbb. 5-2:Main menuFig. 5-2: Main menu5.3 Navigating the menu5.3.1 Selecting the menu and navigating5.3.

SoftwareFemtoJet® 4iEnglish (EN)255.3.3 Selecting parametersPrerequisites•A submenu with parameters is selected.5.3.4 Changing a parameter valuePrereq

OperationFemtoJet® 4iEnglish (EN)266Operation6.1 Switching on or off the Microinjector6.1.1 Switching on the Microinjector1. Take off the injection tu

OperationFemtoJet® 4iEnglish (EN)276.2.2 Switching off the standby modePrerequisites• The display shows STANDBY.1. Take off the injection tube.2. Pres

OperationFemtoJet® 4iEnglish (EN)286.3.3 Possible sources of error - cell inflates or burstsThe injected volume is too large.1. Decrease the injection

OperationFemtoJet® 4iEnglish (EN)296.4 Setting the compensation pressure pc The compensation pressure depends on the surface tension, the viscosity of

Table of contentsFemtoJet® 4iEnglish (EN)3Table of contents1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

OperationFemtoJet® 4iEnglish (EN)306.7 Setting the injection mode6.7.1 Setting the automatic injection mode During the automatic injection mode, the s

OperationFemtoJet® 4iEnglish (EN)31 Press the inject key.The injection movement is triggered on the micromanipulator.The inject key is disabled durin

OperationFemtoJet® 4iEnglish (EN)326.12 Calling up saved injection parametersThe program keys are predefined with exemplary parameter sets for standar

OperationFemtoJet® 4iEnglish (EN)336.13.2 Changing the saved injection parameters 1. Change the injection parameters.2. Keep the prog 1 or prog 2 key

OperationFemtoJet® 4iEnglish (EN)346.14.5 Illumination function – Switching on/off the display illumination1. Press the menu enter key.2. Select the I

OperationFemtoJet® 4iEnglish (EN)356.15 Inserting the capillary into the capillary holderPrerequisites• Grip head 0• Capillary with an outer diameter

TroubleshootingFemtoJet® 4iEnglish (EN)367 Troubleshooting7.1 General errors7.2 Error messages7.2.1 Error 01 – 10 Symptom/message Cause RemedyNo liqui

TroubleshootingFemtoJet® 4iEnglish (EN)377.2.2 Error 11 – 18Error #06 • The key pad is faulty.  Switch the device off and back on again. If the erro

TroubleshootingFemtoJet® 4iEnglish (EN)38Error #13 • The compressor does not work or is faulty. Switch the device off and back on again. If the erro

TroubleshootingFemtoJet® 4iEnglish (EN)397.2.3 Error 19 – 38Symptom/message Cause RemedyError #19 • System error Switch the device off and back on ag

Table of contentsFemtoJet® 4iEnglish (EN)44.4 Connecting an external device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 204.

TroubleshootingFemtoJet® 4iEnglish (EN)407.2.4 Error 39 – 40Symptom/message Cause RemedyWarning #39 • Capillary is broken. Insert a new capillary.•In

MaintenanceFemtoJet® 4iEnglish (EN)418 Maintenance8.1 Replacing the sealings in the grip head You must replace any damaged o-rings.8.1.1 Removing the

MaintenanceFemtoJet® 4iEnglish (EN)428.1.2 Inserting the o-rings and the sealing washer8.2 Replacing the sealing in the adapter for Femtotips You must

MaintenanceFemtoJet® 4iEnglish (EN)438.3 Replacing fusesThe fuse holder is located between the mains connection socket and the mains power switch. The

MaintenanceFemtoJet® 4iEnglish (EN)441. Wipe the painted parts and the aluminum surfaces with a cloth and mild detergent.2. Polish with a dry cloth.8.

Technical dataFemtoJet® 4iEnglish (EN)459 Technical data9.1 Mode of operation9.2 Weight/dimensions9.3 Power supply9.4 Interfaces9.4.1 USB9.4.2 RS 232M

Technical dataFemtoJet® 4iEnglish (EN)469.4.3 Injection time ti9.4.4 Injection pressure pi9.4.5 Compensation pressure pc/pw9.4.6 Rinsing pressureTime

Technical dataFemtoJet® 4iEnglish (EN)479.4.7 Accuracy9.5 Ambient conditions6000 hPa87.02 PSI±0.20 %2500 hPa36.26 PSI±0.20 %1000 hPa14.50 PSI±0.10 %10

Transport, storage and disposalFemtoJet® 4iEnglish (EN)4810 Transport, storage and disposal10.1 Storage10.2 Decontamination before shipmentIf you are

Transport, storage and disposalFemtoJet® 4iEnglish (EN)4910.4 DisposalIn case the product is to be disposed of, the relevant legal regulations are to

Table of contentsFemtoJet® 4iEnglish (EN)56.12 Calling up saved injection parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326.12

Ordering informationFemtoJet® 4iEnglish (EN)5011 Ordering information11.1 FemtoJet 4i11.2 Accessories11.3 Universal capillary holder and accessoriesOr

IndexFemtoJet® 4iEnglish (EN)51IndexAAmbient conditions ... 47CCapillaryCleaning ...

IndexFemtoJet® 4iEnglish (EN)52prog 1... 32prog 2... 32Possi

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Table of contentsFemtoJet® 4iEnglish (EN)69 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Operating instructionsFemtoJet® 4iEnglish (EN)71 Operating instructions1.1 Using this manual Read this operating manual completely before using the d

Product descriptionFemtoJet® 4iEnglish (EN)82 Product description2.1 Delivery package2.1.1 Delivery package – Accessories2.2 FeaturesThe Microinjector

Product descriptionFemtoJet® 4iEnglish (EN)92.3 Exemplary set-up of a microinjection systemAbb. 2-1:Microinjection system with FemtoJet 4iFig. 2-1: Mi