Eppendorf Mastercycler nexus Bedienungsanleitung

nual® nexusEN)g manual Register your instrument! www.eppendorf.com/myeppendorfMastercycler® nexusOperating manual

Operating instructionsMastercycler® nexusEnglish (EN)101.5 GlossaryFflexlidThe heated lid of the thermal cycler automatically adapts to the used tubes

Technical dataMastercycler® nexusEnglish (EN)100Mastercycler nexus flat, Mastercycler nexus flat ecoSample capacity Flat block, aluminum4 slidesBlock

Technical dataMastercycler® nexusEnglish (EN)101Mastercycler nexus X2, Mastercycler nexus X2eSample capacity Universal block, aluminum96 × 0.1 mL or 0

Technical dataMastercycler® nexusEnglish (EN)102

Ordering InformationMastercycler® nexusEnglish (EN)10314 Ordering Information14.1 Mastercycler nexus 14.1.1 Master variantsOrder no. (International)Or

Ordering InformationMastercycler® nexusEnglish (EN)10414.1.2 Eco variantsOrder no. (International)Order no. (North America)DescriptionMastercycler n

Ordering InformationMastercycler® nexusEnglish (EN)10514.2 Accessories14.3 ConsumablesUse only genuine Eppendorf accessories or accessories approved b

Ordering InformationMastercycler® nexusEnglish (EN)106The twin.tec PCR plates are also available in various frame colors (red, yellow, green, blue). T

Ordering InformationMastercycler® nexusEnglish (EN)10714.4 Recommended auxiliary materialsOrder no. (International)Order no. (North America)Descripti

IndexMastercycler® nexusEnglish (EN)108Index11 … 3 StepCyc (program step) ...53AAccess rights ...

IndexMastercycler® nexusEnglish (EN)109Login ...37Logout ...

Product descriptionMastercycler® nexusEnglish (EN)112 Product description2.1 Main illustration2.1.1 Master variants•Mastercycler nexus• Mastercycler n

IndexMastercycler® nexusEnglish (EN)110Standby...46Status view ...

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Product descriptionMastercycler® nexusEnglish (EN)122.1.2 Master variants with thermoblock (64+32 wells)•Mastercycler nexus X2•Mastercycler nexus GX2A

Product descriptionMastercycler® nexusEnglish (EN)132.1.3 Master variant with flat thermoblockAbb. 2-3: Front and rear view of the Mastercycler nexus

Product descriptionMastercycler® nexusEnglish (EN)142.1.4 Eco variants•Mastercycler nexus eco• Mastercycler nexus gradient eco•Mastercycler nexus SX1e

Product descriptionMastercycler® nexusEnglish (EN)152.1.5 Terminal blockAbb. 2-5: Terminal block Fig. 2-5: Terminal block Only connect devices to the

Product descriptionMastercycler® nexusEnglish (EN)162.1.6 Control panelAbb. 2-6: Control panelFig. 2-6: Control panel1stop keyStop the running program

Product descriptionMastercycler® nexusEnglish (EN)172.1.7 Number pad To enter text, press the key with the desired letter until the respective letter

Product descriptionMastercycler® nexusEnglish (EN)182.3 FeaturesTriple Circuit Technology The Triple Circuit Technology leads to a homogeneous tempera

Product descriptionMastercycler® nexusEnglish (EN)192.3.1 Features of the Mastercycler nexusThermoblock versionThermoblock materialFreely programmable

6331 900.069-04/012014Copyright © 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of Eppendor

Product descriptionMastercycler® nexusEnglish (EN)202.3.2 Control panel and portsControl panel Ethernet port USB portMastercycler nexus gradient X X 2

Product descriptionMastercycler® nexusEnglish (EN)212.3.3 ConsumablePCR tubes (0.1 mL or 0.2 mL)PCR tubes *)(0.5 mL)PCR plates PCR slide (in situ)Mast

Product descriptionMastercycler® nexusEnglish (EN)222.3.4 NetworkingYou can connect and control up to any two eco devices to a master variant of the M

SafetyMastercycler® nexusEnglish (EN)233Safety3.1 Intended useThe Mastercycler nexus is intended for use in research and routine laboratories in the b

SafetyMastercycler® nexusEnglish (EN)243.4 Warnings for intended useBefore using the Mastercycler nexus, read the operating manual and observe the fol

SafetyMastercycler® nexusEnglish (EN)25CAUTION! Risk of burns on thermoblock, heated lid and reaction vessels.The thermoblock, heated lid and reaction

SafetyMastercycler® nexusEnglish (EN)263.5 Warning signs on the deviceThis section explains the warning signs on the Mastercycler nexus.1CAUTIONRisk o

InstallationMastercycler® nexusEnglish (EN)274 Installation4.1 Selecting the locationInformation regarding the dimensions and weight of device compone

InstallationMastercycler® nexusEnglish (EN)284.2 Installing the instrumentThis section describes how to put the device into operation and how to conne

InstallationMastercycler® nexusEnglish (EN)294.2.2 Attaching devices to each otherYou can attach adjacent devices to each other to prevent individual

Table of contentsMastercycler® nexusEnglish (EN)3Table of contents1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

InstallationMastercycler® nexusEnglish (EN)301. Position the devices next to each other.2. Fold out the bracket of the device on the left . Insert th

InstallationMastercycler® nexusEnglish (EN)311. Open the flap (1) to access the USB ports (2).2. Insert the USB device into one of the two ports.Newly

InstallationMastercycler® nexusEnglish (EN)324.2.5.2 Setting the Eco and Term switchesYou must set the Eco and Term switches on all devices depending

OperationMastercycler® nexusEnglish (EN)335 Operation5.1 Initial stepsChecking the correct installationBefore the Mastercycler nexus is commissioned f

OperationMastercycler® nexusEnglish (EN)345.1.1.2 Closing the heated lidPrerequisitesThermoblock loaded with sample tubes or a PCR plate.1. Grip the h

OperationMastercycler® nexusEnglish (EN)355.2 Starting the Mastercycler nexusPrerequisitesThe Mastercycler nexus is properly set up and connected to t

OperationMastercycler® nexusEnglish (EN)36To define the administrator PIN, perform the following steps in the sequence described.After the successful

OperationMastercycler® nexusEnglish (EN)375.3 Logging in and out as a user5.3.1 Logging in as a user To be able to log in with your user name, your ad

OperationMastercycler® nexusEnglish (EN)385.3.2 Changing usersOnly one user can be logged in at a time. Proceed as follows to switch between users:5.3

OperationMastercycler® nexusEnglish (EN)395.4 Overview of software operation5.4.1 Navigation tree overview1 Window titleCurrent level in the navigatio

Table of contentsMastercycler® nexusEnglish (EN)45.3 Logging in and out as a user. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

OperationMastercycler® nexusEnglish (EN)405.4.2 Navigating in the navigation tree5.4.2.1 Opening the nodeYou have three options:5.4.2.2 Closing the no

OperationMastercycler® nexusEnglish (EN)415.4.3.2 Removing the USB storage mediumCaution! The symbol is displayed in the upper right corner while th

OperationMastercycler® nexusEnglish (EN)425.5.1 Creating folders and programs5.5.1.1 Creating a folderProceed as follows:1. Mark the user node.2. Pres

OperationMastercycler® nexusEnglish (EN)435.5.1.2 Creating a new programYou can create programs in the navigation tree on the user node level or on th

OperationMastercycler® nexusEnglish (EN)445.5.2 Copying folders and programs To copy folders or programs into your own user node, proceed as follows.

OperationMastercycler® nexusEnglish (EN)455.6.1 Editing a schedule entryThe following window appears if you choose to display, create or change a sche

OperationMastercycler® nexusEnglish (EN)465.6.2 Displaying the scheduleYou can access the schedule from the Mastercycler Schedule and User Login windo

OperationMastercycler® nexusEnglish (EN)47Switching on the power save modeExiting the power save mode Press the standby key.The device switches to po

OperationMastercycler® nexusEnglish (EN)48

ProgrammingMastercycler® nexusEnglish (EN)496 Programming6.1 Overview of the program editorThe graphic program editor can be used to view and change e

Table of contentsMastercycler® nexusEnglish (EN)58 System management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

ProgrammingMastercycler® nexusEnglish (EN)506.1.2 Structure of the program editorA program consists of a program header and up to 99 program steps tha

ProgrammingMastercycler® nexusEnglish (EN)516.2 Making general settings Each Mastercycler nexus program has a program header where you define the bloc

ProgrammingMastercycler® nexusEnglish (EN)526.2.2 Editing header settingsLidtemp. Heated lid temperature (37°C to 110°C). The set lid temperature is

ProgrammingMastercycler® nexusEnglish (EN)536.3 Editing programsYou can add, edit or delete steps in new (see p. 43) or copied (see p. 44) programs.6.

ProgrammingMastercycler® nexusEnglish (EN)545. Press the Insert softkey.The program step is inserted with standard parameters. You can now insert addi

ProgrammingMastercycler® nexusEnglish (EN)554. Press the OK softkey to accept the settings.The program view appears.5. Press the >> softkey. Pre

ProgrammingMastercycler® nexusEnglish (EN)561. Mark the temperature control step in which you would like to define the gradient.2. Press the Options s

ProgrammingMastercycler® nexusEnglish (EN)576. Press the OK softkey to accept the settings and close the window.The gradient step is symbolized in the

ProgrammingMastercycler® nexusEnglish (EN)586.4 Program templatesThe following table provides an overview of the program templates. The program templa

PCR runMastercycler® nexusEnglish (EN)597PCR run7.1 Loading the thermoblock7.1.1 Selecting the sample tubes7.1.1.1 Approved sample tubes for devices w

Table of contentsMastercycler® nexusEnglish (EN)614 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PCR runMastercycler® nexusEnglish (EN)607.1.1.4 Compatible PCR plate typesThe following PCR plate types are compatible. A selection of the PCR micro t

PCR runMastercycler® nexusEnglish (EN)61Observe the following notes when loading the thermoblock with tubes Place the tubes or strips into the center

PCR runMastercycler® nexusEnglish (EN)627.2.1 Status viewThe status view is automatically displayed immediately after a program is started. It shows t

PCR runMastercycler® nexusEnglish (EN)637.2.1.1 Displaying the status viewProceed as follows:1. Mark the cycler in the navigation tree.2. Press the St

PCR runMastercycler® nexusEnglish (EN)647.3 Interrupting or stopping a program7.3.1 Interrupting a program1. Open status view (see Starting a program

PCR runMastercycler® nexusEnglish (EN)65If the PIN function is switched off or the administrator is logged in, the Recently Used node is located bel

PCR runMastercycler® nexusEnglish (EN)66

System managementMastercycler® nexusEnglish (EN)678 System management8.1 Administrator functionsThe Mastercycler nexus software differentiates between

System managementMastercycler® nexusEnglish (EN)683. Enter the name and PIN of the new user. Re-enter the PIN in the Confirmation: field.4. Optionally

System managementMastercycler® nexusEnglish (EN)698.2.3 Deleting a user accountYou must be logged in as an administrator to be able to delete user acc

Operating instructionsMastercycler® nexusEnglish (EN)71 Operating instructions1.1 Using this manual Read the operating manual before you operate the

System managementMastercycler® nexusEnglish (EN)708.3 System settings To change the system settings, open the System node.Beeper: Activate or deactiv

System managementMastercycler® nexusEnglish (EN)71Schedule Settings: Make schedule settingsUse the Schedule Settings node to configure the schedule vi

System managementMastercycler® nexusEnglish (EN)72System Configuration: Set device-dependent entries for ramp and gradientMaking changes under System

System managementMastercycler® nexusEnglish (EN)738.4 Cycler functionsThe cycler nodes enable you to view and adjust the status of the thermoblock a

System managementMastercycler® nexusEnglish (EN)748.4.1 Cycler system functionsThe System node contains the cycler system level with the following sof

System managementMastercycler® nexusEnglish (EN)758.4.2 Self-testThe self-test function allows you to perform a quick check regarding the following at

System managementMastercycler® nexusEnglish (EN)768.5 Managing and backing up data 8.5.1 Backing up dataThe softkeys in the window above show the pos

System managementMastercycler® nexusEnglish (EN)778.5.2 Exporting and importing programs 8.6 Transferring programs from an older Mastercycler modelPCR

System managementMastercycler® nexusEnglish (EN)78Example Make the desired settings in the header (see Editing header settings on p. 52).1 T = 94.0°

System managementMastercycler® nexusEnglish (EN)79Press the OK softkey.The editor is displayed. Press the >> softkey. Press the Save softkey.Y

Operating instructionsMastercycler® nexusEnglish (EN)81.3 Symbols usedDepiction Meaning 1.2.Actions in the specified order Actions without a specif

System managementMastercycler® nexusEnglish (EN)808.7.1 Setting up a network connectionPrerequisites• Ethernet cable (RJ45, Cat.5), not included in th

System managementMastercycler® nexusEnglish (EN)818.7.2 Setting up e-mail notificationsPrerequisites• Network connection has been set up (see p. 80)•

System managementMastercycler® nexusEnglish (EN)824. Select the data you would like to send and their file type.• Cycler run protocol: Protocol of the

System managementMastercycler® nexusEnglish (EN)834. Select the information that you would like to create a protocol for.• Cycler run protocol entries

System managementMastercycler® nexusEnglish (EN)84

Quick startMastercycler® nexusEnglish (EN)859 Quick start9.1 Login1. Open the list.2. Select the user.3. Confirm your selection.4. Switch to the PIN:

Quick startMastercycler® nexusEnglish (EN)869.2 Creating folders and programs9.3 Copying folders and programs1. Select the user or folder.2. Press the

Quick startMastercycler® nexusEnglish (EN)879.4 Deleting folders and programs9.5 Editing programs1. Mark the folder or program.2. Press the del key.3.

Quick startMastercycler® nexusEnglish (EN)888. Select the program step.9. Press the Insert softkey.10. Enter the temperatures, holding times and numbe

Quick startMastercycler® nexusEnglish (EN)899.6 Stopping and starting programs1. Mark the program.2. Insert the sample tubes.3. Close the heated lid.4

Operating instructionsMastercycler® nexusEnglish (EN)91.4 Abbreviations usedDHCPDynamic Host Configuration Protocol (network)DNSDomain Name System (ne

Quick startMastercycler® nexusEnglish (EN)90

MaintenanceMastercycler® nexusEnglish (EN)9110 Maintenance10.1 Cleaning10.1.1 Cleaning the housing Clean the surfaces of the Mastercycler nexus with

MaintenanceMastercycler® nexusEnglish (EN)9210.1.2 Cleaning the heated lid and the thermoblock1. Open the heated lid.2. Remove contamination on the th

TroubleshootingMastercycler® nexusEnglish (EN)9311 Troubleshooting11.1 General errorsTechnical faults may be triggered by faults such as a power failu

TroubleshootingMastercycler® nexusEnglish (EN)94

Transport, storage and disposalMastercycler® nexusEnglish (EN)9512 Transport, storage and disposal12.1 PackingUse the supplied packing material when p

Transport, storage and disposalMastercycler® nexusEnglish (EN)9612.2 DisposalIn case the product is to be disposed of, the relevant legal regulations

Technical dataMastercycler® nexusEnglish (EN)9713 Technical data13.1 Power supply13.2 Weight/dimensions13.3 Ambient conditionsVoltage 100 V – 130 V, ±

Technical dataMastercycler® nexusEnglish (EN)9813.4 Application parametersMastercycler nexus gradient, Mastercycler nexus gradient ecoSample capacity

Technical dataMastercycler® nexusEnglish (EN)99Mastercycler nexus GSX1, Mastercycler nexus GSX1eSample capacity Silver block96 × 0.1 mL or 0.2 mL PCR