Eppendorf BioSpectrometer basic Bedienungsanleitung PDF herunterladen (Seite 81)

Troubleshooting

Eppendorf BioSpectrometer

basic

English (EN)

• Calculation not

possible

because of

division by zero.

Absorbance

result is zero.

• Calculation

error. Division

by zero.

• The evaluation required dividing by

an absorbance result with the value of

"zero". This is not mathematically

permissible.

Examples: Calculation of a factor at

one-point calibration; calculation of a

260/280 ratio with nucleic acid

measurements.

 Check the reagents and samples used

and repeat the measurement.

Calculation not

possible because of

division by zero.

Absorbance result

or parameter

formula b is zero.

• An absorbance result was divided by

a "zero" value during the evaluation

of a Division type method (Dual

wavelength method group). This is

not mathematically permissible.

 Check the reagents and samples used

and repeat the measurement.

 Do not enter "zero" as a value for the

Formula b parameter.

Problem Cause Solution

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Eppendorf BioSpectrometer basic Bedienungsanleitung Seite 81