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Technical data
Eppendorf BioSpectrometer
®
basic
English (EN)
11.6 Application parameters
Methods Preprogrammed and freely programmable methods for all measuring
and evaluation procedures:
• Absorbance measurements with one or more wavelengths, scans
• Nucleic acids and proteins, OD600, dye methods (parallel
measurement of biomolecule and dye marking)
• Methods with evaluation via factor, standard and standard series
• Dual wavelength procedure with subtraction and division
evaluation
Method-dependent evaluation Absorbance, concentration via factor and standard.
Concentration via standard series:
• Linear regression
• Nonlinear regression (2nd and 3rd degree polynoms)
• Spline evaluation
• Linear interpolation (point-to-point evaluation)
Absorbance calculations via subtraction and division
Additional data for nucleic acids: Ratios 260/280 and 260/230; molar
concentration, total yield
Additional data for dye methods: FOI (frequency of incorporation,
labeling density)
Scans: zoom, peak evaluation
Method memory >100 method programs
Measured value memory and
calibration memory
Memory for >1 000 results with all data of the results evaluation and
standard evaluation, sample number, sample name, date and used
parameter set of the method program.
(The number of saved results depends on the number of saved
methods.)
- Eppendorf BioSpectrometer 1
- Table of contents 3
- 1 Operating instructions 7
- 1.1 Using this manual 7
- 1.3 Symbols used 8
- 1.4 Abbreviations used 9
- Operating instructions 10
- English (EN) 10
- 2 Product description 11
- 2.1 Main illustration 11
- 2.2 Delivery package 11
- 2.3 Features 12
- 3 Safety 13
- 3.1 Intended use 13
- 3.2 User profile 13
- 3.3 Warnings for intended use 13
- 3.3.2 Damage to device 14
- Do not open the device 16
- 4 Installation 17
- 4.1 Preparing installation 17
- 4.2 Selecting the location 17
- 4.4 Connecting the printer 17
- 4.4.2 Thermal printer DPU-414 18
- Installation 20
- 5 Operation 21
- 5.1.1 Entering text 23
- 5.2 Inserting the cuvette 24
- 5.3.2 Measuring procedure 25
- 5.3.2.1 Selecting a method 25
- 5.3.2.2 Checking parameters 26
- 5.3.2.4 Measuring samples 27
- 5.3.2.5 Finalizing the method 27
- Operation 30
- 6 Methods 31
- 6.1 Selecting a method 31
- 6.2.1 Absorbance method group 32
- 6.2.2 Routine method group 33
- 6.2.3 Basic method group 34
- 6.3 Method parameters 35
- 6.4 Method procedure 40
- 6.4.1 Check parameters 41
- 6.4.2 Measure standards 42
- 6.4.3 Measure samples 43
- 6.4.5 Process results 50
- 6.4.7 Print & export 55
- 7 Functions 59
- 7.1.1 Results memory 60
- Confirm with enter 61
- 7.1.4 Device settings 64
- 7.1.5 Device calibration 64
- 7.1.6 Info 65
- Functions 66
- 8 Maintenance 67
- 8.1 Cleaning 67
- 8.3 Checking the device 69
- Maintenance 70
- 8.4 Replacing fuses 73
- 9 Troubleshooting 75
- 9.1 General errors 75
- 9.2 Error messages 76
- 9.3 Result flags 79
- Troubleshooting 82
- 10.1 Transport 83
- 10.2 Storage 83
- 10.3 Disposal 83
- 11 Technical data 85
- 11.1 Power supply 85
- 11.2 Ambient conditions 85
- 11.3 Weight/dimensions 85
- 11.4 Photometric properties 86
- 11.6 Application parameters 87
- Technical data 88
- 12 Evaluation procedure 89
- 12.1 Absorbance values 89
- 12.1.3 Cuvette correction 90
- 12.4 Dilution 92
- YYYXXXcorrXXX 93
- ACFAA u 93
- S, total 94
- 12.6.2 Calculation of the FOI 96
- 12.7 Dual wavelength 97
- Evaluation procedure 98
- 13 Ordering information 99
- Ordering information 100
- Evaluate your manual 104
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