Eppendorf BioPhotometer plus Bedienungsanleitung

BioPhotometer plusOperating manualB 6132 900.017-00/1107

3 SafetyBioPhotometer plus — Operating manual103Safety3.1 Intended useThe intended area of use for the BioPhotometer plus is the research laborator

113 SafetyBioPhotometer plus — Operating manualWARNING!Warning! Risk to health from contaminated device Perform decontamination before storing or

3 Safety12BioPhotometer plus — Operating manual3.3 Application limits3.4 Note on product liabilityIn the following cases, the protection provided i

134 InstallationBioPhotometer plus — Operating manual4 Installation4.1 Preparing installationKeep the transport carton and the packing material fo

4 Installation14BioPhotometer plus — Operating manual4.5 Connect printerYou can connect the Eppendorf thermal printer to the serial interface RS-23

155 OperationBioPhotometer plus — Operating manual5 Operation5.1 Overview of operating controlsAbb. 3: Control panel of the BioPhotometer plus.Fig.

5 Operation16BioPhotometer plus — Operating manual5.2 MethodsThe following methods are available and already preprogrammed ex factory. You can modi

175 OperationBioPhotometer plus — Operating manual5.3 Summary of the measuring procedureThis section contains a summary of the key steps of a measu

5 Operation18BioPhotometer plus — Operating manual5.3.3 Measure1. Open the cuvette shaft by sliding the blue cover back.blank 2. Fill the cuvette w

195 OperationBioPhotometer plus — Operating manual5.3.4 Finalize the method1. Press one of the method group keys to return to the method selection

6132 900.017-02/032012Copyright© 2007 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyrig

5 Operation20BioPhotometer plus — Operating manual5.5 ProteinsYou can measure the protein concentrations directly by measuring in the UV range at 2

215 OperationBioPhotometer plus — Operating manual5.6 Methods with evaluation via standardsFor the following methods you can define an evaluation v

5 Operation22BioPhotometer plus — Operating manual5.7 OD 600With method "OD 600" you can measure the bacteria density via a turbidity mea

235 OperationBioPhotometer plus — Operating manual5.8.3 Frequency of incorporation "FOI"The FOI is a measure for the concentration of the

5 Operation24BioPhotometer plus — Operating manualAs with the nucleic acid measurement without dye measurement (see Nucleic acids on page 19) you c

255 OperationBioPhotometer plus — Operating manualThe dilution is retained for calculating all subsequent sample results until it is overwritten. T

6 Parameter and functionsBioPhotometer plus — Operating manual266 Parameter and functions6.1 ParameterThe methods of the BioPhotometer plus as well

276 Parameter and functionsBioPhotometer plus — Operating manualREGRESSION Selection:Linear and non-linear regressionEvaluation procedure of the mu

6 Parameter and functions28BioPhotometer plus — Operating manual6.1.3 Parameters preprogrammed ex factoryNucleic acids and OD 600ProteinsParameter

296 Parameter and functionsBioPhotometer plus — Operating manualMethod group "dye 550"The unit for the dye is defined permanently with &q

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6 Parameter and functions30BioPhotometer plus — Operating manualassay 340assay 405 and assay 490The parameters correspond to those of method group

316 Parameter and functionsBioPhotometer plus — Operating manual6.2 Functions1. In the method selection press the key function/·/No..A list of gene

7 MaintenanceBioPhotometer plus — Operating manual327 Maintenance7.1 Cleaning1. Switch off the device and disconnect the power plug.2. Wipe down th

337 MaintenanceBioPhotometer plus — Operating manual7.2 Disinfection / Decontamination1. Switch off the device and disconnect the power plug.2. Pri

7 Maintenance34BioPhotometer plus — Operating manual7.5 Check photometerTo check the photometric accuracy and the accuracy of the wavelength Eppend

357 MaintenanceBioPhotometer plus — Operating manual7.5.1 Test procedure1. Open the function PHOTOMETER TEST / NEW MEASUREMENT.2. Select the test f

8 TroubleshootingBioPhotometer plus — Operating manual368 Troubleshooting8.1 Result flagsResult flags Possible cause Remedy0.015 A340¶ Only for nuc

378 TroubleshootingBioPhotometer plus — Operating manual8.2 Error messagesDevice displays with error messages can be exited using Enter.If certain

8 Troubleshooting38BioPhotometer plus — Operating manual8.3 General errorsFault Possible cause RemedyMeasuring results are imprecise.• Shelf life o

399 Transport, storage and disposalBioPhotometer plus — Operating manual9 Transport, storage and disposal9.1 TransportOnly transport the device in

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10 Technical dataBioPhotometer plus — Operating manual4010 Technical data10.1 Power supply10.2 Ambient conditionsNot tropicalized.Protect against d

4110 Technical dataBioPhotometer plus — Operating manual10.5 Photometer10.6 Other technical parametersMeasuring principle Absorption single beam ph

10 Technical data42BioPhotometer plus — Operating manual10.7 Application parametersMethod memory 32 preprogrammed, modifiable method programsMeasur

4311 Evaluation procedureBioPhotometer plus — Operating manual11 Evaluation procedureThis chapter describes the available evaluation procedures in

11 Evaluation procedure44BioPhotometer plus — Operating manual11.2.2 Multi-point calibration: calibration lineFor the evaluation via a calibration

4511 Evaluation procedureBioPhotometer plus — Operating manual11.4 Special evaluation procedures for the dye methods11.4.1 Calculating the factor f

11 Evaluation procedure46BioPhotometer plus — Operating manual11.5 Special evaluation procedures for nucleic acids and protein UVThis section relat

4711 Evaluation procedureBioPhotometer plus — Operating manualCalculation of the molar concentrationApplication: calculation of the molar concentra

12 Ordering information48BioPhotometer plus — Operating manual12 Ordering in formationOrder No. (International)Order No. (North America)Description

5Table of contentsBioPhotometer plus — Operating manualOperating manualTable of contents1 User instructions . . . . . . . . . . . . . . . . . . . .

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Table of contents6BioPhotometer plus — Operating manual7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

71 User instructionsBioPhotometer plus — Operating manual1 User instructions1.1 Using this manualBefore using the device for the first time, pleas

2 Product descriptionBioPhotometer plus — Operating manual82 Product description2.1 Main illustrationAbb. 1: Front and rear viewFig. 1: Front and r

92 Product descriptionBioPhotometer plus — Operating manualEvaluation The BioPhotometer plus converts the measured absorbance values into concentra