Eppendorf BioSpectrometer basic Bedienungsanleitung

nualoSpectrometer® basicEN)g manual Register your instrument! www.eppendorf.com/myeppendorfEppendorf BioSpectrometer® basicOperating manual

Operating instructionsEppendorf BioSpectrometer® basicEnglish (EN)10

Ordering informationEppendorf BioSpectrometer® basicEnglish (EN)100

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Evaluate your manualGive us your feedback.www.eppendorf.com/manualfeedbackYour local distributor: www.eppendorf.com/contactEppendorf AG · 22331 Hambur

11Product descriptionEppendorf BioSpectrometer® basicEnglish (EN)2 Product description2.1 Main illustrationAbb. 2-1: Front and rear viewFig. 2-1: Fron

Product descriptionEppendorf BioSpectrometer® basicEnglish (EN)122.3 FeaturesThe BioSpectrometer basic is a UV/Vis spectrophotometer for measuring liq

13SafetyEppendorf BioSpectrometer® basicEnglish (EN)3 Safety3.1 Intended useThe BioSpectrometer basic is to be used in molecular biology, biochemistry

SafetyEppendorf BioSpectrometer® basicEnglish (EN)143.3.2 Damage to deviceWARNING! Damage due to UV radiation.Microliter cuvettes, e.g., Hellma® TrayC

15SafetyEppendorf BioSpectrometer® basicEnglish (EN)3.4 Information on product liabilityIn the following cases, the designated protection of the devic

SafetyEppendorf BioSpectrometer® basicEnglish (EN)163.5 Safety instructions located on the deviceDepiction Meaning LocationHazard point Follow the op

17InstallationEppendorf BioSpectrometer® basicEnglish (EN)4 Installation4.1 Preparing installation Keep the transport carton and the packing material

InstallationEppendorf BioSpectrometer® basicEnglish (EN)184.4.2 Thermal printer DPU-414Connect the thermal printer DPU-414 to the serial printer conne

19InstallationEppendorf BioSpectrometer® basicEnglish (EN)4.5 Connecting PC or USB stick for data exportYou can connect a FAT 32-formatted USB stick t

6135 900.050-03/052014 Copyright © 2014 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyr

InstallationEppendorf BioSpectrometer® basicEnglish (EN)20

21OperationEppendorf BioSpectrometer® basicEnglish (EN)5 Operation5.1 Overview of operating controlsAbb. 5-1: Control panel of the BioSpectrometer bas

OperationEppendorf BioSpectrometer® basicEnglish (EN)22Key: FunctionKeypad: Enter digits and text.Keys 1 to 9 as well as 0: When entering text, next t

23OperationEppendorf BioSpectrometer® basicEnglish (EN)5.1.1 Entering textYou can enter texts when assigning method names and result units. Restrictio

OperationEppendorf BioSpectrometer® basicEnglish (EN)245.2 Inserting the cuvetteStandard rectangular glass or plastic cuvettes can be inserted in the

25OperationEppendorf BioSpectrometer® basicEnglish (EN)5.3 Summary of the measuring procedure5.3.1 Preparing the measurement1. Switch on the device an

OperationEppendorf BioSpectrometer® basicEnglish (EN)265.3.2.2 Checking parameters5.3.2.3 Measuring the blank and standards Check the parameter setti

27OperationEppendorf BioSpectrometer® basicEnglish (EN)5.3.2.4 Measuring samples5.3.2.5 Finalizing the method5.3.2.6 Optional: process results The sa

OperationEppendorf BioSpectrometer® basicEnglish (EN)285.3.2.7 Printing and exporting1. Compose data packets for all samples or for selected samples.2

29OperationEppendorf BioSpectrometer® basicEnglish (EN)5.3.3 Important measurement instructionsCheck for each measurement:• For plastic cuvettes: How

3Table of contentsEppendorf BioSpectrometer® basicEnglish (EN)Table of contents1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . .

OperationEppendorf BioSpectrometer® basicEnglish (EN)30

31MethodsEppendorf BioSpectrometer® basicEnglish (EN)6 Methods6.1 Selecting a methodMethods and method templates are delivered preprogrammed. The meth

MethodsEppendorf BioSpectrometer® basicEnglish (EN)32Tab. 6-2: Softkeys in method selectionCopied or cut methods can be added to a different folder un

33MethodsEppendorf BioSpectrometer® basicEnglish (EN)6.2.2 Routine method groupThe methods for the Routine group are preprogrammed as fixed methods. T

MethodsEppendorf BioSpectrometer® basicEnglish (EN)34Dye labels• For dye-labeled biomolecules: Concentration determination of the biomolecule (nucleic

35MethodsEppendorf BioSpectrometer® basicEnglish (EN)6.2.4 Advanced method groupDual wavelength• Measurement at two wavelengths and evaluation of the

MethodsEppendorf BioSpectrometer® basicEnglish (EN)36Unit Selection:mg/mL | μg/mL | ng/mL | pg/mL | μg/μL | mg/dL | μmol/mL | nmol/mL | pmol/mL | pmol

37MethodsEppendorf BioSpectrometer® basicEnglish (EN)Factor Value input:Factor.Limit: max. of 6 digits including decimal point.Factor for converting a

MethodsEppendorf BioSpectrometer® basicEnglish (EN)38Correct A260 1 Selection:on | offOnly for the Dye labels method group.Correction of the influence

39MethodsEppendorf BioSpectrometer® basicEnglish (EN)FOI Selection:none | dye/kb | pmole/μgOnly for the Dye labels method group.Display of the FOI in

Table of contentsEppendorf BioSpectrometer® basicEnglish (EN)46 Methods. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

MethodsEppendorf BioSpectrometer® basicEnglish (EN)406.4 Method procedureA method procedure is composed of a maximum of 5 steps. The currently active

41MethodsEppendorf BioSpectrometer® basicEnglish (EN)6.4.1 Check parametersSoftkeys• [Page dn] and [Page up]: Change between 1 to 3 parameter list pag

MethodsEppendorf BioSpectrometer® basicEnglish (EN)426.4.2 Measure standardsThe first standard to be measured is marked on the display. After the blan

43MethodsEppendorf BioSpectrometer® basicEnglish (EN)6.4.3 Measure samplesThe sample key is used for measuring your samples consecutively. Blank resul

MethodsEppendorf BioSpectrometer® basicEnglish (EN)44Enter dilutionSoftkeys• [Clear dil.]: Delete values for sample dilution.• [OK]: Confirm sample di

45MethodsEppendorf BioSpectrometer® basicEnglish (EN)Result image with dilution and ID6.4.4 Measure samples: Results displaysThis section contains a d

MethodsEppendorf BioSpectrometer® basicEnglish (EN)46Scan Results display:• Scan (graph with absorbance wavelength display)• Navigate between the meas

47MethodsEppendorf BioSpectrometer® basicEnglish (EN)Proteins direct UVResults display:• Concentration result with absorbance at the measuring wavelen

MethodsEppendorf BioSpectrometer® basicEnglish (EN)48Dye labels Results display:• Concentration results with absorbance at the measuring wavelength of

49MethodsEppendorf BioSpectrometer® basicEnglish (EN)Advanced main groupDual wavelength Results display:• Concentration result: calculated from Acalc.

5Table of contentsEppendorf BioSpectrometer® basicEnglish (EN)11 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

MethodsEppendorf BioSpectrometer® basicEnglish (EN)506.4.5 Process resultsThe sample measurement is followed by two optional steps in the method seque

51MethodsEppendorf BioSpectrometer® basicEnglish (EN)After changes have been made, you can exit the current mode using the two softkeys at right:• [Sa

MethodsEppendorf BioSpectrometer® basicEnglish (EN)526.4.6 Process results: OptionsZoomPress the [Zoom] softkey and select one of the following versio

53MethodsEppendorf BioSpectrometer® basicEnglish (EN)More calculationsPress the [More calc.] softkey.Nucleic acids method group:• After the molar mass

MethodsEppendorf BioSpectrometer® basicEnglish (EN)54Peak detectionPress the [Peaks] softkey. For the peak detection you can alternate between two cri

55MethodsEppendorf BioSpectrometer® basicEnglish (EN)6.4.7 Print & exportIn the last optional method step, you can assemble data packets for all s

MethodsEppendorf BioSpectrometer® basicEnglish (EN)56Export to USB stick1. Connect a FAT 32-formatted USB stick to the USB port 4 (see Main illustrati

57MethodsEppendorf BioSpectrometer® basicEnglish (EN)Select data packetsBased on the method and parameter setting, only the available data packets are

MethodsEppendorf BioSpectrometer® basicEnglish (EN)58

59FunctionsEppendorf BioSpectrometer® basicEnglish (EN)7 Functions7.1 Functions of the User main groupWith the function key or the [Function] softkey,

Table of contentsEppendorf BioSpectrometer® basicEnglish (EN)6

FunctionsEppendorf BioSpectrometer® basicEnglish (EN)607.1.1 Results memory In the right column, select the method for which you would like to call u

61FunctionsEppendorf BioSpectrometer® basicEnglish (EN)7.1.2 General method parameters If you would like to print or export results, select the data

FunctionsEppendorf BioSpectrometer® basicEnglish (EN)62When programming a method of the Dye labels or Proteins direct UV method groups, you can access

63FunctionsEppendorf BioSpectrometer® basicEnglish (EN)Tab. 7-2: Parameter in General Method ParameterParameter ExplanationProteins These parameters a

FunctionsEppendorf BioSpectrometer® basicEnglish (EN)647.1.3 Absorbance spectra library7.1.4 Device settings7.1.5 Device calibrationInformation on che

65FunctionsEppendorf BioSpectrometer® basicEnglish (EN)7.1.6 InfoThe Copyright menu item contains license information on the Open Source software.

FunctionsEppendorf BioSpectrometer® basicEnglish (EN)66

67MaintenanceEppendorf BioSpectrometer® basicEnglish (EN)8 Maintenance8.1 Cleaning1. Wipe down the surfaces with a cloth moistened with a mild cleanin

MaintenanceEppendorf BioSpectrometer® basicEnglish (EN)688.1.1 Cleaning the cuvette shaft coverIf you would like not only to clean the directly access

69MaintenanceEppendorf BioSpectrometer® basicEnglish (EN)8.2 Disinfection/Decontamination1. Clean the device with a mild cleaning agent before the dis

7Operating instructionsEppendorf BioSpectrometer® basicEnglish (EN)1 Operating instructions1.1 Using this manual Read this operating manual completel

MaintenanceEppendorf BioSpectrometer® basicEnglish (EN)70Abb. 8-1: Inside of the filter box lid (sample)Fig. 8-1: Inside of the filter box lid (sample

71MaintenanceEppendorf BioSpectrometer® basicEnglish (EN)8.3.1.1 Checking photometric accuracy1. Select the Spectrometer unit function in the Device c

MaintenanceEppendorf BioSpectrometer® basicEnglish (EN)72Have the filter recertified by Eppendorf Service after 2 years.8.3.1.2 Checking wavelength sy

73MaintenanceEppendorf BioSpectrometer® basicEnglish (EN)8.3.2 Device self-testThe frequency of the automatic self-test (approx. 1 minute) can be set

MaintenanceEppendorf BioSpectrometer® basicEnglish (EN)748.5 Decontamination before shipmentIf you are shipping the device to the authorized Technical

75TroubleshootingEppendorf BioSpectrometer® basicEnglish (EN)9 Troubleshooting9.1 General errorsError Possible cause RemedyMeasuring results are impre

TroubleshootingEppendorf BioSpectrometer® basicEnglish (EN)769.2 Error messagesYou can exit device displays with error messages using the [OK] softkey

77TroubleshootingEppendorf BioSpectrometer® basicEnglish (EN)The following parameter values are not defined in General Method Parameter:• When opening

TroubleshootingEppendorf BioSpectrometer® basicEnglish (EN)78Calculation not possible because of division by zero. Absorbance result or Formula "

79TroubleshootingEppendorf BioSpectrometer® basicEnglish (EN)9.3 Result flagsWarnings and error messages for results are displayed in the bottom right

Operating instructionsEppendorf BioSpectrometer® basicEnglish (EN)81.3 Symbols usedDepiction Meaning1.2.Actions in the specified order Actions with

TroubleshootingEppendorf BioSpectrometer® basicEnglish (EN)80The coefficient of determination for the regression evaluation of the standard series is

81TroubleshootingEppendorf BioSpectrometer® basicEnglish (EN)• Calculation not possible because of division by zero. Absorbance result is zero.• Calcu

TroubleshootingEppendorf BioSpectrometer® basicEnglish (EN)82

83Transport, storage and disposalEppendorf BioSpectrometer® basicEnglish (EN)10 Transport, storage and disposal10.1 Transport Use the original packag

Transport, storage and disposalEppendorf BioSpectrometer® basicEnglish (EN)84

85Technical dataEppendorf BioSpectrometer® basicEnglish (EN)11 Technical data11.1 Power supply11.2 Ambient conditionsDo not expose to direct sunlight.

Technical dataEppendorf BioSpectrometer® basicEnglish (EN)8611.4 Photometric properties11.5 Further technical parametersMeasuring principle Single bea

87Technical dataEppendorf BioSpectrometer® basicEnglish (EN)11.6 Application parametersMethods Preprogrammed and freely programmable methods for all m

Technical dataEppendorf BioSpectrometer® basicEnglish (EN)88

89Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)12 Evaluation procedureThis chapter describes the evaluation procedures available in

9Operating instructionsEppendorf BioSpectrometer® basicEnglish (EN)1.4 Abbreviations usedAAbsorbanceDNADeoxyribonucleic aciddsDNADouble-stranded DNADy

Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)9012.1.3 Cuvette correctionAll absorbance values which are used for result calculation

91Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)12.2 Evaluation with factor or standardC = calculated concentration.A = absorbance.F

Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)9212.4 DilutionIn the measure samples method step. entered dilutions are considered in

93Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)12.5 Special evaluation procedures for nucleic acids and protein UVThis section cove

Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)9412.5.3 Conversion to molar concentrations and nucleic acid quantitiesThe conversion

95Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)12.5.4 Calculating the factor for protein in "General Method Parameter"Thi

Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)9612.6.2 Calculation of the FOIAs a value for the ratio of dye molecules to the number

97Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)12.7 Dual wavelengthFor methods of the Dual Wavelength group absorbances that were m

Evaluation procedureEppendorf BioSpectrometer® basicEnglish (EN)98

99Ordering informationEppendorf BioSpectrometer® basicEnglish (EN)13 Ordering informationOrder no. (International)Order no. (North America)Description