Eppendorf InjectMan 4 Bedienungsanleitung PDF herunterladen (Seite 64)

100

Operation

InjectMan

English (EN)

6.11.2 Executing the Inject function

Prerequisites

• A FemtoJet is connected.

• A lower limit (Z-axis Limit) has been defined.

1. Press the joystick key.

• The capillary is moved to the side and to the lower limit at the injection angle.

• The injection is carried out automatically.

• The capillary is moved back to the starting position.

1 Position the capillary

2 Trigger an automatic injection

3 Injection

4 Automatic movement to the starting

position

5 Capillary

6 Search level

7 Z-axis Limit

8 Cell

If the Search+limit parameter is activated you can inject cells with different

heights using the same injection parameters.

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InjectMan 1
Table of contents 3
2.1 Delivery package 9
2.1.1 Tools 9
2.1.2 Accessories 9
2.2 Features 9
2 Product description 9
2.3 Main illustration 10
2.3.1 Motor module 11
2.3.2 Control board 12
Abb. 2-4:Control board - rear 13
2.4 Control panel 14
2.5.1 Dynamic range 15
2.5 Joystick 15
2.5.3 Joystick key functions 16
2.6 Speed ranges 17
3.1 Intended use 18
3.2 Warnings for intended use 18
4.3 Mounting overview 21
4.3.1 Module (X, Y, Z) 22
4.3.2 Z-module holder 23
4.3.3 Slider 23
4.3.4 Angle head 24
4.3.5 Swivel joint 25
4.4 Mounting the motor module 25
4.4.1 Mounting the Z-module 26
4.4.2 Mounting the Y-module 26
4.4.3 Mounting the X-module 27
4.4.4 Mounting the angle head 28
4.6.1 Inserting Femtotips 30
4.8 Aligning the motor module 31
4.8.1 Aligning the height 31
4.8.2 Aligning the depth 31
4.8.3 Aligning the width 32
4.8.4 Aligning the angle head 32
4.9.1 Microscope and adapter 32
4.9.2 Motor module 33
4.9.3 Angle head 33
4.13.1 First set-up wizard 40
4.14.4 Connecting two devices 44
5 Software 45
5.1 Display 45
5.1.1 Application display 45
5.1.2 Display of coordinates 46
5.1.3 Menu display 47
5.2 Applications 47
5.2.1 Application parameter 48
5.2.3 Application – ICSI 49
5.3 Main menu 51
 Save with Enter 52
6.3 Defining the start screen 54
6.4 Replacing the capillary 54
6.5 Changing the sample 56
6.6 Changing the speed range 56
6.7 Capillary positions 57
6.7.1 Saving a position 57
 Press the joystick key 58
6.8 Vertical limits 59
Upper Limit 60
6.9 Horizontal limit 61
6.10 Speed function 62
6.11 Inject function 63
6.12 Step injection function 65
6.13 Home function 66
6.13.5 Setting the offset 67
6.14 Clean function 68
6.15 PiezoXpert function 70
6.16 Installation function 71
6.16.4 Module parameters 72
6.17 Function function 72
6.17.4 Executing User default 73
6.18 Softkeys function 74
6.18.2 Executing Softkeys 75
6.18.3 Executing Joystick key 76
6.19 Change appl. function 76
6.20 Service function 77
6.21.1 Perform reset 78
7 Troubleshooting 79
7.1 General errors 79
7.1.1 Motor module 79
7.1.2 Capillary 79
7.1.4 Joystick 80
7.1.5 Software and parameters 80
7.2 Error messages 80
7.2.1 Warnings 80
7.2.2 Error 81
8 Maintenance 82
8.1 Replacing fuses 82
8.2 Cleaning 82
8.3 Service and maintenance 83
9 Technical data 84
9.1 Power supply 85
9.2 Interfaces 85
9.3 Ambient conditions 85
10.1 Storage 86
10.3 Transport 86
10.4 Disposal 87
11 Ordering Information 88
11.1 InjectMan 4 88
11.3 Tools for InjectMan 4 89
11.4 Microscope adapter 89
11.7 Capillaries 91
11.9 CellTram and accessories 92
11.10 FemtoJet 92
11.11 FemtoJet express 93
11.13 PiezoXpert 94
Evaluate your manual 100
Give us your feedback 100

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